Incidence of pleuro-pneumonia-like organisms (P.P.L.O.) in man.
نویسنده
چکیده
P.P.L.O. cultures were grown on a soft nutrient agar (0-8 per cent.) with horse serum (15 per cent.), and substances inhibiting the growth of undesired bacteria (thallium acetate 1: 2000 (w/v) and penicillin 50 I.U./ ml.) (Edward, 1947). Identification was by observation of the morphological characteristics of colonies and growth specificities, and the microscopic picture was studied with a phase-contrast microscope. The material investigated in female cases consisted mainly of vaginal discharge, as positive results had been found more often in P.P.L.O. cultures from the vagina than from urethra or cervix. Trichomonas vaginalis was detected by Wright's staining method and in wet specimens (in one drop of 0-85 per cent. NaCl). The approximate count of neutrophils in the microscopic field was also determined.
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عنوان ژورنال:
- The British journal of venereal diseases
دوره 37 شماره
صفحات -
تاریخ انتشار 1961